Abstract:
Background: The aim of the present study was to investigate the effect of resveratrol on cytokine levels and oxidative stress in intestinal ischemia/reperfusion (I/R) injuries.
Methods: To induce intestinal I/R, the superior mesenteric artery was occluded for 30 min and then reperfused for 150 min or 24 h. The therapeutic effects of resveratrol on the damage from intestinal I/R were investigated using an isolated organ bath, along with oxidant/antioxidant and inflammatory factors such as glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), interleukin-(IL)-1 beta, and tumor necrosis factor alpha (TNF-alpha).
Results: I/R control animals demonstrated severe deterioration of smooth muscle motor function as a significant decrease in potassium chloride- and acetylcholine-induced a contractile responses; high oxidative stress as an increase in lipid peroxidation and a decrease in GSH level; and an increase of MPO, IL-1 beta, and TNF-alpha activity. Pretreatment of animals with resveratrol restored intestinal dysfunction; reduced elevated MDA, MPO, IL-1 beta, and TNF-alpha levels; and reversed the depleted intestine GSH levels after both 150 min and 24 h reperfusion periods.
Conclusions: The results indicated that resveratrol can reverse the effects of disrupted smooth muscle contractility, probably because of its antioxidant and anti-inflammatory effects on MPO, IL-1 beta, and TNF-alpha activities.
