Culture-dependent diversity of boron-tolerant bacteria from boron mine tailings pond and solid wastes

Abstract

Boron is a vital micronutrient for the growth of plants and different organisms. However, boron may be toxic to living cells at high concentrations. Nevertheless, microorganisms can be grown in harsh conditions such as environments with higher boron concentrations. It is also known that boron-tolerant microorganisms have significant biotechnological potentials. In this study, it is aimed to isolate microorganism from a boron containing place in Kirka, Eskisehir using culture-dependent methods. After extracting genomic DNAs, Amplified Ribosomal DNA Restriction Analysis (ARDRA) profiles were constructed with HaeIII, MspI, HinfI-HpaII and AluI-CfoI. Sequence analyses were performed by chain-terminating method. Consensus sequences were completed and similarities were determined by RDP Release 11. According to ARDRA, 10 profiles and five different species such as Pseudomonas sp., Enterecoccus sp., Acinetobacter sp., Bacillus sp. and Halomonas sp. were identified.