Investigation of XRCC1 Arg399Gln, Arg280His, and Arg194Trp polymorphisms effects on the induction of micronucleus by Aflatoxin B1 in in vitro

Abstract

Aflatoxin B1 (AFB1) is a genotoxic mycotoxin that can contaminate a wide variety of foods. The aim of this study was to examine the associations between XRCC1 Arg399Gln, Arg280His, and Arg184Trp polymorphisms and the frequencies of spontaneous and AFB1-induced DNA damage in peripheral blood lymphocytes from 100 healthy individuals in Turkish population. In vitro cytokinesis-blocked micronucleus (CMBN) assay was used to detect the spontaneous and AFB1-induced DNA damage. Genotyping of XRCC1 Arg399Gln, Arg280His, and Arg194Trp polymorphisms were carried out by using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay. Treatment of the lymphocytes with AFB1 slightly increased (for Arg399Gln 0.73 +/- 0.4 vs. 0.40 +/- 0.26, Arg280His 0.80 +/- 0.4 vs. 0.41 +/- 0.28) the overall frequencies of micronucleus (MN) when compared to untreated cultures. No results were obtained for Arg194Trp polymorphism. Obtained overall results do not correspond to any significant (P < 0.05) association between XRCC1 Arg399Gln, Arg280His, and Arg194Trp Polymorphisms and MN frequencies. The results of this study indicate that Arg399Gln, Arg280His, and Arg194Trp polymorphisms do not play an important role in human sensitivity to the genotoxic effects of AFB1.