Sahin, B (Sahin, Birgutay) [1] ; Aygün, A (Aygun, Aysenur) [1] ; Gündüz, H (Gunduz, Hulya) [1] ; Sahin, K (Sahin, Kubilay) [1] ; Demir, E (Demir, Enes) [1] ; Akocak, S (Akocak, Suleyman) [1] , [2] ; Sen, F (Sen, Fatih) [1]

Abstract

The study utilizes monodisperse platinum nanoparticles (Pt NPs) biosynthesized from Punica granatum crusts as anti-tumor agents on the human breast cancer cell line, MCF-7. The obtained Pt NPs were fully characterized using the UV-vis spectrum (UV-vis), transmission electron microscopy (TEM), Xray diffraction (XRD), field emission scanning electron microscopy (FESEM) and Fourier transformation infrared spectroscopy (FTIR). Effectiveness of the Pt NPs was determined by cell viability, propidium iodide staining test, flow cytometry and comet tests on the MCF-7 cancer cell line. Cell survival percentage was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The biosynthesized monodisperse platinum nanoparticles inhibited MCF-7 proliferation with an IC50 of 17.84 mu g/ml after 48 h of incubation. Propidium iodide staining demonstrated that the monodisperse Pt NPs induced apoptosis by means of molecular DNA fragmentation. (C) 2017 Elsevier B.V. All rights reserved.