Enhanced -fructofuranosidase biosynthesis by Rhodotorula glutinis using Taguchi robust design method

Abstract

The aim of this study was to produce -fructofuranosidase enzyme by Rhodotorula glutinis SO28, using sugar beet (Beta vulgaris) as carbon source due to its high sucrose content and easy availability. -Fructofuranosidase production was carried out in submerged fermentation. Taguchi orthogonal array (OA) design of experiment (DOE) method was employed for optimization process of -fructofuranosidase production by R. glutinis SO28. An OA layout of L16 was constructed with five influential factors on -fructofuranosidase biosynthesis namely, carbon source (sugar beet), initial pH, incubation temperature, agitation speed and incubation time. The average results of -fructofuranosidase yield obtained from the determined 16 batches were processed with Minitab((R)) 16.2.3 software at larger is better as quality character. The results showed that the maximum -fructofuranosidase activity was obtained as 21.11 +/- 0.47U/mL, which was close to the predicted result (21.78 +/- 0.43U/mL). Consequently, sugar beet can be suggested as an economical substrate for -fructofuranosidase production. Besides, use of Taguchi DOE enhanced enzyme activity about 3-fold when compared with unoptimized condition.