Adıyaman Üniversitesi Kurumsal Arşivi

MicroRNA 211 expression is upregulated and associated with poor prognosis in colorectal cancer: a case-control study

Basit öğe kaydını göster

dc.contributor.author Sümbül, Ahmet Taner
dc.contributor.author Göğebakan, Bülent
dc.contributor.author Bayram, Süleyman
dc.contributor.author ve diğerleri...
dc.date.accessioned 2023-01-20T10:56:31Z
dc.date.available 2023-01-20T10:56:31Z
dc.date.issued 2015
dc.identifier.issn 1010-4283
dc.identifier.uri http://dspace.adiyaman.edu.tr:8080/xmlui/handle/20.500.12414/4349
dc.description.abstract Increasingly more evidence support the role of the microRNAs (miRNA) in tumorigenesis. The role of up/downregulation microRNA-211 (miR-211) during human tumorigenesis is still contentious and may exhibit tissue-specific regulatory manner, but the exhaustive mechanisms underlying its pro/anti-oncogenic effects remain to be unknown. Sixty-six patients that were diagnosed and operated with colorectal cancer (CRC) and sixty-five healthy cases that were age and sex compatible with them were included in our study. miRNA was isolated from the formalin-fixed paraffin-embedded (FFPE) tissues of all cases. The expression level of miR-211 in matched normal and tumor tissues of CRC group and healthy group was evaluated using a quantitative real-time RT-PCR (qRT-PCR). Based on the average miR-211 levels, two groups of low or high expression were formed in CRC group. Correlation of the patients' clinicopathological factors and survival was also analyzed. No statistically significant differences were found in miR-211 levels among tumorous and normal tissues of CRC patient group (P = 0.59). Also, no statistically significant correlation was determined between clinicopathological factors and miR-211 expression level in CRC group. However, miR-211 expression levels between the CRC group and the healthy group were determined to be of statistical significance (P < 0.0001). There were 33 (50 %) CRC patients that expressed low levels of miR-211 and 33 (50 %) CRC patients that expressed high levels of miR-211. A median survival between low levels of miR-211 group and high levels of miR-211 group was evaluated via Kaplan-Meier, and the difference was of statistical significance (P = 0.035). The univariate analysis of the factors that may affect survival indicated invasion depth (P = 0.063), lymphovascular invasion (P = 0.011), perineural invasion (P = 0.009), and miR-211 expression level (P = 0.041) presence to be effective. In the multivariate analysis of these factors with overall survival, only miR-211 expression level (P = 0.01) was effective on overall survival. Our results suggest for the first time that miR-211 expressed more in CRC patients than in healthy group could be a new prognostic biomarker in order to predict survival. Independent studies are needed to validate our findings in a larger series, as well as in cancer of different tissues. tr
dc.language.iso en tr
dc.publisher Sage tr
dc.subject Colorectal cancer tr
dc.subject microRNA tr
dc.subject miR-211 tr
dc.subject Biomarker tr
dc.title MicroRNA 211 expression is upregulated and associated with poor prognosis in colorectal cancer: a case-control study tr
dc.type Article tr
dc.contributor.authorID 0000-0002-5573-906X tr
dc.contributor.authorID 0000-0002-7087-8615 tr
dc.contributor.department Baskent Univ, Dept Med Oncol, Adana, Turkey tr
dc.contributor.department Mustafa Kemal Univ, Dept Biol & Genet, Antakya, Turkey tr
dc.identifier.endpage 9709 tr
dc.identifier.issue 12 tr
dc.identifier.startpage 9703 tr
dc.identifier.volume 36 tr
dc.source.title Tumor Biology tr


Bu öğenin dosyaları:

Bu öğe aşağıdaki koleksiyon(lar)da görünmektedir.

Basit öğe kaydını göster