The effect of solid surface vitrification (ssv) versus classic vitrification technique on survive rate of in vitro produced bovine blastocysts

Abstract

The aim of this study was to compare Solid Surface Vitrification (SSV) technique and classic vitrification technique in in vitro produced 8 days old bovine blastocysts. Cryopreservation of mammalian embryo has great importance for genetic resources conservation, embryo transfer, veterinary and clinical reproductive biotechnology and animal assisted reproductive technologies. Immature oocytes were maturated then fertilized with frozen-thawed bull semen and cultured until blastocyst stage in commercial sequential culture medium for 8 days. Blastocysts were vitrified in two different groups as SSV and classic vitrification and non-vitrified blastocysts were used as control group. After vitrification, vitrified blastocysts were warmed and cultured for 1 day. For this aim, blastocyst viability rate and median cell number were investigated. The blastocyst viability rate that vitrified by classic vitrification (34.8%) were found to be lower than those vitrified by SSV (82.6%1 and control group blastocysts (100%). However, median cell numbers of vitrified-warmed blastocysts were found higher in SSV (124) than classic vitrification (104). Median cell number of control group was detected as 213. As a result blastocyst viability rate and median cell number in SSV group was higher than classic vitrification group, there was a significant difference between SSV and classic vitrification group (p<0.05).